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Related Experiment Video

Updated: Jun 11, 2026

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Mouse Oocyte Microinjection, Maturation and Ploidy Assessment

Published on: July 23, 2011

High-efficiency somatic reprogramming induced by intact MII oocytes.

Hui Yang¹, Linyu Shi, Shenghua Zhang

¹Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.

|July 7, 2010

Summary

This summary is machine-generated.

Researchers developed a highly efficient method for somatic cell reprogramming by combining nuclear transfer and oocyte injection. This new technique generates pluripotent embryonic stem cells for potential therapeutic applications and mechanistic studies.

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Area of Science:

Reproductive Biology
Stem Cell Biology
Developmental Biology

Background:

Somatic cell reprogramming is crucial for understanding pluripotency but remains inefficient.
Existing methods like nuclear transfer, cell fusion, and transcription factor expression have limitations.

Purpose of the Study:

To develop a simple and highly efficient somatic cell reprogramming strategy.
To generate tetraploid (4N) and triploid (3N) embryonic stem (ES) cells.
To investigate the potential of these ES cells for transplantation and mechanistic studies.

Main Methods:

Injection of cumulus cell nuclei into intact MII oocytes.
Activation of reconstructed oocytes.
Generation and characterization of 4N and 3N ES cell lines.
In vivo differentiation and transplantation studies in immunocompetent mice.

Main Results:

80% of reconstructed embryos developed to the blastocyst stage.
30% of reconstructed oocytes yielded 4N ES cell lines.
Generated 4N and 3N ES cells expressed pluripotent markers.
ES cells differentiated into three embryonic germ layers in vivo.
Engrafted ES cells produced histocompatible differentiated cells in mice.

Conclusions:

A simple, highly efficient reprogramming procedure was established.
This method provides a system for studying somatic reprogramming mechanisms.
ES cells derived from this strategy may offer a source for genetically tailored tissues for transplantation.