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Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...

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Related Experiment Video

Updated: Jun 11, 2026

A Microfluidic Chip for ICPMS Sample Introduction
11:16

A Microfluidic Chip for ICPMS Sample Introduction

Published on: March 5, 2015

Liquid chromatography on chip.

Karine Faure1

  • 1Laboratoire des Sciences Analytiques, Université de Lyon, Villeurbanne, France. karine.faure@univ-lyon1.fr

Electrophoresis
|July 7, 2010
PubMed
Summary
This summary is machine-generated.

Microscale liquid chromatography (LC) is advancing rapidly for miniaturized analytical systems. Recent breakthroughs focus on novel stationary phases and instrumental designs for LC on chip, enhancing separation power in microdevices.

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Last Updated: Jun 11, 2026

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Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Liquid chromatography (LC) is a cornerstone separation technique in analytical sciences.
  • Miniaturization of LC for micro-Total Analysis Systems (µTAS) presents significant challenges.
  • Electrophoretic techniques are common in miniaturized devices, but LC faces hurdles in downsizing.

Purpose of the Study:

  • To review recent advancements in LC on chip technology.
  • To highlight the development and implementation of novel stationary phases in microchannels.
  • To present instrumental progress and discuss driving forces (pressure, electrical fields) for microscale LC.

Main Methods:

  • Review of literature on LC on chip development over the past five years.
  • Analysis of novel stationary phase materials and their integration into microfluidic devices.
  • Examination of instrumental advancements, including pressure-driven and electrokinetic systems.

Main Results:

  • Significant breakthroughs have been achieved in the conception and application of LC on chip.
  • Novel stationary phases are being developed and successfully implemented in microchannels.
  • Diverse driving forces, including pressure and electrical fields, are utilized with varying application ranges.

Conclusions:

  • LC on chip technology has seen substantial progress, overcoming previous downsizing challenges.
  • Innovations in stationary phases and instrumentation are key to the advancement of miniaturized LC.
  • The development of LC on chip is crucial for the future of micro-Total Analysis Systems.