Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developmental regulation of gap junction gene expression during mouse embryonic development.

M Nishi1, N M Kumar, N B Gilula

  • 1Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.

Developmental Biology
|July 1, 1991
PubMed
Summary

Gap junction gene expression, including alpha 1, beta 1, and beta 2, was analyzed in mouse embryos and cells. Alpha 1 is the earliest detected transcript, crucial for early development and stem cell function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transperineal approach alone as minimally invasive surgery for pelvic recurrence of rectal cancer after abdominoperineal resection.

Techniques in coloproctology·2025
Same author

Transanal total mesorectal excision alone as minimally invasive surgery for metachronous rectal cancer after a Hartmann's procedure.

Techniques in coloproctology·2022
Same author

Interaction of Eating Status and Dietary Variety on Incident Functional Disability among Older Japanese Adults.

The journal of nutrition, health & aging·2022
Same author

Score-Based and Nutrient-Derived Dietary Patterns Are Associated with Depressive Symptoms in Community-Dwelling Older Japanese: A Cross-Sectional Study.

The journal of nutrition, health & aging·2019
Same author

Oral health: The Sakata model.

British dental journal·2018
Same author

Albumin, Hemoglobin, and the Trajectory of Cognitive Function in Community-Dwelling Older Japanese: A 13-Year Longitudinal Study.

The journal of prevention of Alzheimer's disease·2017

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Gap junctions are essential for intercellular communication.
  • Understanding gap junction gene expression is key to comprehending embryonic development and cell differentiation.

Purpose of the Study:

  • To investigate the expression patterns of three gap junction genes (alpha 1, beta 1, beta 2) during mouse embryonic development.
  • To examine the modulation of these genes during F9 teratocarcinoma cell differentiation and in embryonic stem (ES) cells.

Main Methods:

  • Analysis of gap junction gene expression in pre- and postimplantation mouse embryos.
  • Study of gene expression during organogenesis and F9 cell differentiation.
  • Investigation of gene expression in cultured embryonic stem cells.

Related Experiment Videos

Main Results:

  • Alpha 1 transcript is the earliest detected gap junction RNA, appearing at the 4-cell stage and increasing with development.
  • All three gap junction genes show developmental expression during embryogenesis and organogenesis.
  • Gap junction gene expression is modulated during F9 cell differentiation, representing the first evidence of this in a single cell type.
  • Only alpha 1 gap junction gene product was detected in ES cells, consistent with its presence in the inner cell mass.

Conclusions:

  • Gap junction gene expression is dynamically regulated during mouse embryonic development and cell differentiation.
  • The alpha 1 gene plays a significant role in early embryogenesis and in undifferentiated stem cells.
  • Differential expression of gap junction genes suggests distinct roles in embryonic development, organogenesis, and adult organ function.