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High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
Published on: October 19, 2013
Three novel Notch genes in zebrafish: implications for vertebrate Notch gene evolution and function
1Department of Developmental Neuroscience, BMC, Uppsala University, Box 587, 751 23, Uppsala, Sweden.
Development Genes and Evolution
|July 8, 2010
Summary
Researchers identified three new zebrafish Notch genes, revealing distinct expression patterns and suggesting varied roles in embryonic development and signaling specificity. This advances understanding of Notch gene evolution and function.
Area of Science:
- Developmental Biology
- Molecular Genetics
Background:
- Notch genes encode transmembrane receptors crucial for animal development.
- These receptors interact with multiple signal transduction pathways.
Purpose of the Study:
- To isolate and characterize novel zebrafish Notch genes.
- To investigate the expression patterns and potential functions of these genes in vertebrate development.
Main Methods:
- Isolation of cDNA fragments for three novel zebrafish Notch genes: Notch1b, Notch5, and Notch6.
- Analysis of gene sequences to infer signaling specificities.
- Examination of gene expression patterns during zebrafish embryogenesis.
Main Results:
- Notch1b identified as a second zebrafish Notch1 gene, potentially with distinct signaling specificity.
- Notch5 and Notch6 represent novel vertebrate Notch gene subfamilies.
- Differential expression observed: Notch1b absent in presomitic mesoderm, Notch5 shows metameric expression, and Notch6 is excluded from the nervous system.
Conclusions:
- The distinct expression patterns of Notch1b, Notch5, and Notch6 suggest specialized roles in gastrulation, somitogenesis, neurogenesis, and other developmental processes.
- Findings provide insights into Notch gene evolution and functional diversification in vertebrates.
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03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...