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Biomechanical Testing of Murine Tendons
Published on: October 15, 2019
Technical note: an R program for automating bone cross section reconstruction
Adam D Sylvester1, Evan Garofalo, Christopher Ruff
1Department of Human Evolution, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, Leipzig D-04103, Germany. adam.sylvester@eva.mpg.de
Abstract:
Many recent studies have used long bone cross-sectional geometric properties in various comparative analyses. Methods have been described for reconstructing diaphyseal cross sections from external molds and biplanar radiographs that produce accurate results (within 5% of true values on average). The manual image processing required, however, is both time and labor intensive. A new freely available program developed here for the computational freeware, R, automates much of the process. This study compares cross-sectional properties calculated using the new R program to those from peripheral quantitative CT (pQCT) and the original manual method. We find that the R program works aswell as the original manual image processing for most cross sections eliminates the chance for entry errors at several steps and greatly speeds up data collection.
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