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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
[A real-time polymerase chain reaction-based test system for quantitation of Gumboro disease virus]
Voprosy Virusologii
|July 9, 2010
Abstract:
A real-time polymerase chain reaction-based test system for quantitation of infectious bursal disease (Gumboro disease) virus was developed. The reaction parameters were analyzed, which affected the linear relationship of a C1 depentanizer to the quantity of cDNA. The use of specific primers for reverse transcription was shown tohave some advantage over that of random hexanucleotides.

