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Evaluation of three different adhesive systems using a bacterial method to develop secondary caries in vitro
Luciana Cardoso Espejo1, Maria Regina Lorenzetti Simionato, Lucia Pereira Barroso
1Department of Operative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
American Journal of Dentistry
|July 9, 2010
Summary
Clearfil SE Bond demonstrated superior protection against secondary root caries in vitro. This dental adhesive system effectively minimized lesion formation compared to other tested systems, suggesting better dentin interaction.
Area of Science:
- Dental Materials Science
- Cariology
- Restorative Dentistry
Background:
- Secondary root caries remains a significant challenge in restorative dentistry.
- Dental adhesive systems play a crucial role in preventing marginal leakage and subsequent caries.
- Understanding the anticariogenic potential of different adhesive systems is vital for clinical practice.
Purpose of the Study:
- To evaluate the efficacy of three distinct dental adhesive systems in preventing secondary root caries formation.
- To compare the anticariogenic effects of Clearfil SE Bond, Xeno III, and Scotchbond Multi-Purpose Plus in a standardized in vitro model.
- To assess lesion development in the presence of a standardized interfacial gap within a filled cavity model.
Main Methods:
- Forty human molars were prepared with standardized Class V cavities and restored with resin composite.
- Teeth were divided into four groups: three adhesive systems (Clearfil SE Bond, Xeno III, Scotchbond Multi-Purpose Plus) and a negative control.
- A standardized interfacial gap was created, and teeth were subjected to a cariogenic challenge using Streptococcus mutans.
- Lesion depth and extension were quantified using light microscopy and specialized software.
Main Results:
- Clearfil SE Bond significantly reduced wall lesion extension and depth compared to Xeno III and Scotchbond Multi-Purpose Plus.
- No significant differences in wall lesion extension were observed between Xeno III and Scotchbond Multi-Purpose Plus.
- All tested adhesive systems exhibited similar performance regarding outer lesion depth.
- SEM analysis corroborated the statistical findings, indicating minimal caries development with Clearfil SE Bond.
Conclusions:
- Clearfil SE Bond demonstrates superior performance in inhibiting secondary root caries formation compared to the other evaluated adhesive systems.
- The interdiffusion zone of Clearfil SE Bond likely contributes to enhanced interaction with dentin, providing protection against recurrent caries.
- Further clinical studies are warranted to confirm these in vitro findings regarding the anticariogenic potential of dental adhesives.
