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Related Experiment Video

Updated: Jun 11, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

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Published on: March 11, 2018

[Uncoupling protein 2 combats oxidative damage to human sperm].

Zhi-Hong Fu¹, Yong-Hong Zhou, Wen-Jie Zhu

¹Department of Reproductive Healthcare, Shenzhen Maternity and Child Healthcare Hospital, Shenzhen, Guangdong 518000, China. fzhlxylxw@yahoo.com.cn

Zhonghua Nan Ke Xue = National Journal of Andrology

|July 9, 2010

Summary

Uncoupling protein 2 (UCP2) protects human sperm from oxidative stress by reducing reactive oxygen species (ROS) production. Lower UCP2 levels correlate with increased oxidative damage and reduced sperm motility.

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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation

Published on: January 22, 2020

Area of Science:

Reproductive biology
Cellular stress response
Biochemistry

Background:

Germ cells are highly susceptible to oxidative stress, a key factor in male infertility.
Uncoupling protein 2 (UCP2) is known to protect various cells from apoptosis induced by reactive oxygen species (ROS).

Purpose of the Study:

To investigate the protective role of UCP2 against oxidative damage in human sperm.
To assess the correlation between UCP2 expression, oxidative stress markers, and sperm quality.

Main Methods:

Analysis of 97 human semen samples using computer-assisted sperm analysis (CASA) to categorize sperm motility.
Detection of UCP2 mRNA expression in sperm via RT-PCR.
Quantification of malondialdehyde (MDA) as a marker of ROS in sperm suspensions.

Main Results:

A significant negative correlation (r = -0.633, P < 0.01) was observed between UCP2 mRNA expression and MDA content.
Sperm with lower motility exhibited significantly reduced UCP2 mRNA levels and elevated MDA content (P < 0.01).

Conclusions:

UCP2 plays a crucial protective role in human sperm against oxidative stress.
UCP2 mitigates oxidative damage by diminishing ROS production, thereby preserving sperm quality and function.