Related Experiment Video
Updated: Jun 11, 2026
12:23
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Host intraspatial selection of infectious bronchitis virus populations
Rodrigo A Gallardo1, Vicky L van Santen, Haroldo Toro
1Department of Pathobiology, College of Veterinary Medicine, 264 Greene Hall, Auburn University, Auburn, AL 36849, USA.
Avian Diseases
|July 9, 2010
Summary
Infectious bronchitis virus (IBV) rapidly changes within chickens after vaccination. Different virus subpopulations emerge, with specific types favoring distinct tissues like the reproductive tract.
Area of Science:
- Veterinary Virology
- Molecular Epidemiology
Background:
- Arkansas (Ark)-type infectious bronchitis virus (IBV) vaccine subpopulations with distinct S gene sequences are selected in chicken upper respiratory tracts.
- This suggests rapid positive selection of distinct IBV subpopulations by the host environment.
Purpose of the Study:
- To investigate how replicating IBV populations change during host invasion due to distinct tissue environments.
- To identify the selection pressures exerted by different tissues on IBV populations.
Main Methods:
- 15-day-old chickens were inoculated with an Ark-type IBV vaccine via ocular and nasal routes.
- S1 gene sequences of IBV in tear fluid, trachea, and reproductive tract were characterized post-inoculation.
Main Results:
- The original vaccine's predominant IBV phenotype became minor or undetectable after replication.
- Five new predominant IBV populations (C1-C5) with distinct nonsynonymous changes were detected.
- Phenotype C1 showed a significantly higher incidence in the reproductive tract compared to the trachea.
Conclusions:
- IBV undergoes intraspatial variation during host invasion, with distinct tissue microenvironments exerting selective pressure.
- The S1 glycoprotein's amino acid changes suggest a phenotypic shift during invasion.
- Tissue-specific selection leads to the emergence of dominant IBV genotypes/phenotypes within the host.
