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Hydrogen peroxide in human blood.

S D Varma1, P S Devamanoharan

  • 1Department of Ophthalmology, University of Maryland School of Medicine & Dentistry, Baltimore 21201.

Free Radical Research Communications
|January 1, 1991
PubMed
Summary

Researchers discovered significant hydrogen peroxide (H2O2) levels in human and rat blood and plasma. Whole blood contained higher H2O2 concentrations than plasma, opening new research avenues for oxygen radical-related diseases.

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Area of Science:

  • Biochemistry
  • Physiology

Background:

  • Hydrogen peroxide (H2O2) is an important reactive oxygen species.
  • Its presence and levels in blood and plasma are not well-established.

Purpose of the Study:

  • To quantify hydrogen peroxide levels in human and rat blood and plasma.
  • To investigate the distribution of H2O2 between whole blood and plasma.

Main Methods:

  • Analysis of blood and plasma samples from humans and rats.
  • Deproteinization using trichloroacetic acid.
  • Quantification using a radio-isotopic technique measuring peroxide-dependent decarboxylation of 1-14C-alpha-ketoacids and 14CO2 liberation.

Main Results:

  • Micromolar levels of H2O2 were detected in both plasma and whole blood.
  • Whole blood consistently showed substantially greater H2O2 concentrations than plasma.
  • These findings were consistent across both human and rat samples.

Conclusions:

  • This study demonstrates for the first time the presence of significant H2O2 quantities in blood.
  • The results suggest a novel area of research concerning diseases linked to in vivo oxygen radical generation.

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