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Super-resolution Imaging of Proteus mirabilis Biofilm by Expansion Microscopy
07:10

Super-resolution Imaging of Proteus mirabilis Biofilm by Expansion Microscopy

Published on: July 18, 2025

Rapidly spreading CTX-M-type beta-lactamase-producing Proteus mirabilis in Japan

Akiko Kanayama1, Takako Iyoda, Kaoru Matsuzaki

  • 1Department of Infection Control and Prevention, Faculty of Medicine, Toho University, 4-16-20, Omori-nishi, Ota-ku, Tokyo 143-0015, Japan.

International Journal of Antimicrobial Agents
|July 9, 2010
PubMed

Insights

Extended-spectrum beta-lactamase (ESBL)-producing Proteus mirabilis, common in Japan, show high CTX-M-type prevalence. These bacteria exhibit reduced susceptibility to fluoroquinolones, necessitating ongoing monitoring.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • Increased isolation of extended-spectrum beta-lactamase (ESBL)-producing Proteus mirabilis reported in Japan.
  • ESBL-producing bacteria pose a significant public health threat due to antimicrobial resistance.

Purpose of the Study:

  • Determine the prevalence of ESBL-producing P. mirabilis in Japan.
  • Characterize the genotype of these isolates.
  • Assess antimicrobial susceptibility patterns, particularly to fluoroquinolones.

Main Methods:

  • Analysis of 74 P. mirabilis isolates from 54 Japanese hospitals (March-October 2006).
  • Detection of ESBL genes using molecular methods.
  • Antimicrobial susceptibility testing, including ciprofloxacin.
  • Pulsed-field gel electrophoresis (PFGE) for genetic relatedness analysis.

Main Results:

  • 37.8% (28/74) of P. mirabilis isolates were ESBL-producers.
  • bla(CTX-M-2) was the predominant ESBL gene (27 isolates).
  • ESBL-producers showed significantly higher non-susceptibility to ciprofloxacin (89.3%) compared to non-producers (23.9%).
  • PFGE revealed 17 clusters among ESBL-producers, with evidence of clonal spread in the Tokyo area.

Conclusions:

  • High prevalence of CTX-M-type beta-lactamase-producing P. mirabilis in Japan.
  • These resistant strains exhibit reduced susceptibility to fluoroquinolones.
  • Potential clonal spread highlights the need for continuous surveillance.

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