Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An immune-associated mitochondrial DNA variant with sex differences reveals a putative novel microprotein called MASL.

Immunity & ageing : I & A·2026
Same author

Mediterranean diet adherence is associated with mitochondrial microproteins Humanin and SHMOOSE; potential role of the Humanin-Nox2 interaction in cardioprotection.

Frontiers in nutrition·2026
Same author

DNMT1 Facilitates the Progression of MASLD by Impeding Transcription Mediated by HNF4α and PPARα.

Clinical and molecular hepatology·2026
Same author

Mitochondrial-derived microproteins in lung disease: insights and implications.

American journal of physiology. Lung cellular and molecular physiology·2026
Same author

Integrating Perspectives on Aging: From Mechanistic Causes to Therapeutic Interventions.

BMB reports·2026
Same author

Mitochondrial DNA Variation in the Aging Human Cerebral Cortex and Cerebellum.

Aging cell·2025

Related Experiment Video

Updated: May 11, 2026

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
11:58

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Published on: June 29, 2018

A new DAF-16 isoform regulates longevity.

Eun-Soo Kwon1, Sri Devi Narasimhan, Kelvin Yen

  • 1Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.

Nature
|July 9, 2010
PubMed
Summary
This summary is machine-generated.

The insulin/IGF-1 signalling pathway uses different DAF-16 isoforms to control longevity and stress responses in C. elegans. These isoforms cooperate for fine-tuned regulation of biological processes.

More Related Videos

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
07:25

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Published on: December 9, 2022

Related Experiment Videos

Last Updated: May 11, 2026

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
11:58

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Published on: June 29, 2018

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
07:25

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Published on: December 9, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Aging Research

Background:

  • The insulin/IGF-1 signalling (IIS) pathway regulates fundamental biological processes, including metabolism and longevity.
  • DAF-16, the sole Caenorhabditis elegans homologue of forkhead box O (FOXO) transcription factors, is a key mediator of IIS.
  • While the DAF-16a isoform is known to influence longevity, stress response, and dauer diapause, the mechanisms underlying DAF-16's functional specificity remain incompletely understood.

Purpose of the Study:

  • To identify and characterize novel DAF-16 isoforms involved in IIS-mediated processes.
  • To elucidate how distinct DAF-16 isoforms achieve functional specificity in regulating diverse biological outcomes.
  • To investigate the cooperative mechanisms and regulatory roles of different DAF-16 isoforms.

Main Methods:

  • Identification of a new DAF-16 isoform, DAF-16d/f.
  • Analysis of functional cooperation between DAF-16 isoforms.
  • Assessment of differential tissue enrichment and kinase modulation of isoforms.
  • Examination of distinct and overlapping target gene regulation.
  • Promoter-swapping experiments to evaluate promoter and coding region contributions.

Main Results:

  • A novel isoform, DAF-16d/f, was identified and shown to be important for longevity regulation.
  • DAF-16 isoforms functionally cooperate to modulate IIS-mediated processes.
  • Isoforms exhibit differential tissue enrichment, preferential modulation by upstream kinases, and regulate distinct/overlapping target genes.
  • Both the promoter and coding regions of DAF-16 are crucial for its function.

Conclusions:

  • Distinct DAF-16 isoforms in C. elegans, analogous to multiple FOXO genes in mammals, utilize isoform-specific mechanisms to fine-tune IIS-mediated processes.
  • Functional cooperation and differential regulation of DAF-16 isoforms provide a mechanism for achieving specificity in complex biological pathways.
  • Understanding isoform-specific functions is critical for comprehending the multifaceted roles of the IIS pathway in aging and stress response.