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Updated: Jun 11, 2026
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Effect of different polymerization methods on the cytotoxicity of dental composites
Nilufer Celebi Beriat1, Ahmet Atila Ertan, Senay Canay
1Hacettepe University, School of Dental Technology, Ankara, Turkey.
Objectives:
The aim of this study was to compare the cytotoxic effects of various dental composites polymerized with two different curing units.
Methods:
Disc-shaped test samples of composites Filtek Z250, Filtek A110, Filtek P60, Filtek Supreme, and SDI Rok were polymerized using one quartz tungsten halogen (QTH) and one light emitting diode (LED) light curing unit (LCU), namely Optilux 501 (QTH) and Elipar Freelight 2 (LED). L-929 mouse fibroblast cultures (3x10(4) cells/ml) were incubated with the samples in 96 well culture plates for evaluation after 8, 24, 48, 72 h. At the end of each period, the cells were counted and examined under a light microscope, and a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was performed. The degree of cytotoxicity for each sample was determined according to the reference value represented by the cells in a control group (a culture without sample).
Results:
A significant 3 factor interaction occurred among LCUs, composites, and time factors (P<.005). In general, the test materials cured with the LED LCU demonstrated higher cell survival rates when compared with those cured with halogen LCUs.
Conclusions:
This study shows that polymerization of dental composites with a light emitting diode LCU positively influences the L-929 mouse fibroblast cell viability.
