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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jun 11, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Wavelength-dependent differential interference contrast microscopy: multiplexing detection using nonfluorescent

Yong Luo¹, Wei Sun, Yan Gu

¹Ames Laboratory-United States Department of Energy and Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.

Analytical Chemistry

|July 10, 2010

Summary

This summary is machine-generated.

Differential interference contrast (DIC) microscopy enables multiplexing detection of nanoparticles. This technique uses unique spectral contrasts at specific wavelengths for identifying multiple nanoparticle types simultaneously in complex biological samples.

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Area of Science:

Nanotechnology
Microscopy
Biophysics

Background:

Plasmonic nanoparticles offer unique optical properties for imaging.
Differential interference contrast (DIC) microscopy visualizes nanostructures based on refractive index differences.
Previous work established wavelength-dependent contrast for single nanoparticle identification.

Purpose of the Study:

To investigate the feasibility of multiplexing detection using DIC microscopy.
To determine if different nanoparticle types exhibit unique spectral signatures in DIC.
To evaluate DIC microscopy's advantages for multiplexed nanoparticle analysis in biological settings.

Main Methods:

Systematic investigation of 19 nanoparticle types (varying materials and sizes) using DIC microscopy.
Measurement of DIC contrast spectra at multiple illumination wavelengths.
Comparison of DIC multiplexing with other non-fluorescence techniques like dark field microscopy and surface-enhanced Raman scattering.

Main Results:

Each nanoparticle type demonstrated a unique DIC contrast spectrum.
Multiplexing detection was achieved by measuring contrasts at two or more specific wavelengths.
Four distinct nanoparticle types were successfully differentiated on a cell membrane using DIC.
High-contrast imaging of both nanoparticles and cellular structures was achieved simultaneously.

Conclusions:

DIC microscopy is suitable for multiplexed detection of nanoparticles.
The unique spectral contrast of nanoparticles in DIC allows for simultaneous identification.
DIC microscopy offers advantages over other techniques for multiplexed nanoparticle imaging in biological environments.