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Updated: Jun 11, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
Furan based cyclic homo-oligopeptides bind G-quadruplex selectively and repress c-MYC transcription
Tani Agarwal1, Saumya Roy, Tushar Kanti Chakraborty
1Proteomics and Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR, New Delhi 110 007, India.
Abstract:
We demonstrate the synthesis and selective binding of two novel furan based tricyclic homo-oligopeptides to G-quadruplex and using Real Time PCR show its repressive effect on c-MYC transcription. CD spectroscopy and FRET melting studies show that these ligands can induce G-quadruplex structures in the G rich 22 mer c-MYC DNA sequence and further stabilize the structure. Using real time polymerase chain reaction we observed that up to 70% downregulation of c-MYC transcripts upon ligand treatment in HeLa cells.
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