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Updated: Jun 11, 2026
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
Published on: August 26, 2013
Development of fluorescent array based on sol-gel/chitosan encapsulated acetylcholinesterase and pH sensitive
Gulsiye Ozturk1, Karl-Heinz Feller, Kay Dornbusch
1Faculty of Arts and Sciences, Department of Chemistry, University of Dokuz Eylul, Izmir, Turkey. gulsiye.ozturk@deu.edu.tr
Abstract:
A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane/chitosan matrix via a microarrayer. The constructed tetraethoxysilane/chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 × 10(-8) M, with a detection limit of 0.27 × 10(-8) M.
