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Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

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Separation of fructooligosaccharides using zeolite fixed bed columns.

Raquel Cristine Kuhn¹, Francisco Maugeri Filho

¹Laboratory of Bioprocess Engineering, Food Engineering Department, P.O. Box 6121, University of Campinas, 13083-862 Campinas, SP, Brazil. raquelk@fea.unicamp.br

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|July 10, 2010

Summary

Y zeolites enhance fructooligosaccharide (FOS) separation. Optimized conditions using Ba2+-exchange Y zeolites improve chromatographic separation of FOS mixtures, aiding in analysis.

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Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

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Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

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Published on: September 6, 2019

Area of Science:

Carbohydrate Chemistry
Separation Science
Analytical Chemistry

Background:

Y zeolites show potential for improving chromatographic separation of mono-, di-, and oligosaccharides.
Fructooligosaccharides (FOS) are important carbohydrates with various applications.
Enzymatic production of FOS yields mixtures including kestose (GF2), nystose (GF3), and frutofuranosyl nystose (GF4).

Purpose of the Study:

To investigate the chromatographic separation of fructooligosaccharides (FOS) using a column packed with zeolite.
To optimize separation conditions for FOS mixtures using Ba2+-exchange Y zeolites.
To determine the effects of temperature, injection volume, superficial velocity, and eluent composition on separation efficiency.

Main Methods:

Fructooligosaccharides were produced enzymatically from Rhodotorula sp.
Separation and analysis were performed using ion exchange chromatography with pulsed amperometric detection (HPAEC-PAD).
A fractional factorial design was employed to investigate the effects of operational parameters on separation efficiency using Ba2+-exchange Y zeolites.

Main Results:

Optimal separation conditions were identified as 60% ethanol eluent, 50°C temperature, 0.1 cm/min superficial velocity, and 2.55% injection volume, using two columns in series.
High separation efficiencies were achieved for various sugar pairs, including oligosaccharide-fructose (1.00) and fructose-sucrose (1.23).
The study demonstrated the effectiveness of Ba2+-exchange Y zeolites for FOS separation.

Conclusions:

Ba2+-exchange Y zeolites provide an effective method for the chromatographic separation of fructooligosaccharides.
Optimized parameters significantly enhance the separation efficiency of FOS mixtures.
This method holds promise for the analysis and purification of oligosaccharides.