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Updated: Jun 11, 2026

Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
Published on: July 19, 2007
[Inhibition of panaxynol on experimental delayed type hypersensitivity]
Min Hong1, Jie Zheng, Liang Wang
1School of Phamacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. hongmin72@hotmail.com
Objective:
To evaluate the effect of panaxynol (PAN) on delayed type hypersensitivity and possible mechanism.
Method:
Allergic contact dermatitis (ACD) was induced by DNCB as a delayed type hypersensitivity (DTH) model to observe effect of PAN on auricle inflammation including pathological injury. Proliferation of T lymphocytes was induced by ConA and measured by MTf method. IFN-gamma secretion of splenocyte induced by ConA was detected by ELISA.
Result:
The swelling degree of auricle and pathological injury in ACD mice was reduced significantly by treated with PAN in induction phase. Proliferation of T lymphocytes induced by ConA in vitro was inhibited significantly by PAN, By contrast, no detectable effect was observed in resting splenocyte. IFN-y induced by ConA in splenocytes was inhibited markedly by PAN from 10 micromol x L(-1) and from 6 h.
Conclusion:
The results showed that DTH was inhibited by PAN mainly in induction phase and this effect may be related with the inhibition on T lymphocytes proliferation and secretion of IFN-gamma.
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