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Updated: Jun 11, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Synthesis, characterization, and studies on DNA binding of the complex Fe(Sal(2)dienNO(3).H(2)O)
Cheng-Yong Zhou1, Yan-Bo Wu, Pin Yang
1Department of Chemistry, Changzhi University, Changzhi, Shanxi, 046011, PR China. zcy826@126.com
Abstract:
A new metal complex, Fe(Sal(2)dienNO(3).H2O) (where Sal is salicylaldehyde and dien is diethylenetriamine), has been synthesized and characterized. The interactions between the Fe(III) complex and calf thymus DNA has been investigated using UV and fluorescence spectra, viscosity, thermal denaturation, and molecular modeling. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. The experimental results show that the mode of binding of the complex to DNA is classical intercalation and the complex can cleave pBR322 DNA.
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