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Related Experiment Video

Updated: Jun 11, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

Streptococcus suis outbreak investigation using multiple-locus variable tandem repeat number analysis.

Wei Li¹, Changyun Ye, Huaiqi Jing

¹State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

Microbiology and Immunology

|July 13, 2010

Summary

A novel Multiple Locus Variable number tandem repeat Analysis (MLVA) method effectively sub-typed Streptococcus suis ST7 strains, revealing a common origin for two distinct outbreaks. This technique offers superior discrimination compared to PFGE for epidemiological tracking.

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Last Updated: Jun 11, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

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Published on: July 15, 2011

Area of Science:

Microbiology
Epidemiology
Molecular typing

Background:

Two Streptococcus suis ST7 outbreaks occurred in China in 1998 and 2005.
Previous methods like PFGE showed indistinguishable ST7 isolates, necessitating a more discriminatory typing technique.

Purpose of the Study:

To develop and evaluate a novel Multiple Locus Variable number tandem repeat Analysis (MLVA) method for sub-typing Streptococcus suis serotype 2 strains.
To apply this MLVA method to analyze Chinese ST7 isolates from the 1998 and 2005 outbreaks.

Main Methods:

Development of a novel MLVA scheme for typing S. suis serotype 2.
Sub-typing of 144 ST7 S. suis isolates using the developed MLVA method.
Comparison of MLVA discriminatory power against PFGE.

Main Results:

The MLVA method sub-typed 144 ST7 isolates into 34 distinct MLVA types.
Isolates from the 1998 outbreak shared MLVA types with isolates from the 2005 Sichuan outbreak, indicating a common origin.
The MLVA scheme demonstrated greater discriminatory power than PFGE.

Conclusions:

The developed MLVA method is highly effective for sub-typing S. suis ST7 strains.
The findings suggest a shared origin for the pathogens involved in the two Chinese outbreaks.
MLVA is a valuable tool for epidemiological investigations, including source identification and spread monitoring of S. suis infections.