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Updated: Jun 11, 2026
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
Published on: July 12, 2017
Comparison of two dimensional electrophoresis mouse colon proteomes before and after knocking out Aquaporin 8
Sameh Magdeldin1, Huiping Li, Yutaka Yoshida
1Department of Structural Pathology Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, 1-757 Asahimachi-dori, Japan. samehmagd@med.niigata-u.ac.jp
Abstract:
Aquaporin (AQP) family plays a fundamental role in transmembrane water and small solutes movement. Within this family, aquaporin 8 (AQP8), showed to be widely distributed in the digestive system especially colon. To investigate the possible protein alterations involved in AQP8 regulation and trafficking, we extensively compared between wild type and AQP8 knockout mouse colon using semi-quantitative fluorescence- stained two dimensional gel electrophoresis (2-DE) coupled with nano LC-Ms/Ms. Our analysis revealed identification and regulation of 21 proteins, most notably, actin-related family which suggests its possible involvement in regulating AQP8 secretory vesicles migration to be integrated as a cell membrane protein.
Insights
Aquaporin 8 (AQP8) protein regulation in the colon was studied. Actin-related proteins were identified, suggesting a role in AQP8 transport to the cell membrane for water and solute movement.
Area of Science:
- Molecular Biology
- Cell Biology
- Physiology
Background:
- The aquaporin (AQP) family is crucial for water and solute transport across cell membranes.
- Aquaporin 8 (AQP8) is notably abundant in the digestive system, particularly the colon.
Purpose of the Study:
- To investigate protein alterations affecting AQP8 regulation and trafficking in the mouse colon.
- To identify proteins involved in the cellular transport of AQP8.
Main Methods:
- Comparative proteomic analysis using semi-quantitative fluorescence-stained two-dimensional gel electrophoresis (2-DE) and nano LC-MS/MS.
- Analysis of colon tissue from wild-type and AQP8 knockout mice.
Main Results:
- Identified and quantified 21 differentially regulated proteins between wild-type and AQP8 knockout mouse colons.
- Highlighted the significant regulation of actin-related proteins.
Conclusions:
- Actin-related proteins likely play a role in regulating the migration of AQP8-containing secretory vesicles.
- These findings suggest a mechanism for integrating AQP8 into the cell membrane for its function.
