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A genomic diagnostic tool for human endometrial receptivity based on the transcriptomic signature
Patricia Díaz-Gimeno1, José A Horcajadas, José A Martínez-Conejero
1Fundación IVI-Instituto Universitario IVI, University of Valencia, and Instituto de Investigación, Sanitaria del Hospital Clinico de Valencia, Valencia, Spain.
Objective:
To create a genomic tool composed of a customized microarray and a bioinformatic predictor for endometrial dating and to detect pathologies of endometrial origin. To define the transcriptomic signature of human endometrial receptivity.
Design:
Two cohorts of endometrial samples along the menstrual cycle were used: one to select the genes to be included in the customized microarray (endometrial receptivity array [ERA]), and the other to be analyzed by ERA to train the predictor for endometrial dating and to define the transcriptomic signature. A third cohort including pathological endometrial samples was used to train the predictor for pathological classification.
Setting:
Healthy oocyte donors and patients.
Patient(S):
Healthy fertile women (88) and women with implantation failure (5) or hydrosalpinx (2).
Intervention(S):
Human endometrial biopsies.
Main Outcome Measure(S):
The gene expression of endometrial biopsies.
Result(S):
The ERA included 238 selected genes. The transcriptomic signature was defined by 134 genes. The predictor showed a specificity of 0.8857 and sensitivity of 0.99758 for endometrial dating, and a specificity of 0.1571 and a sensitivity of 0.995 for the pathological classification.
Conclusion(S):
This diagnostic tool can be used clinically in reproductive medicine and gynecology. The transcriptomic signature is a potential endometrial receptivity biomarkers cluster.