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Updated: Jun 11, 2026
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Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
Published on: November 28, 2025
PBSH: a new improved cardiac preservation solution in comparison with three clinically proven solutions
C L Corps1, M S Attia, D Potts
1Department of Hepatobiliary and Transplant Surgery, St. James's University Teaching Hospital, Leeds, West Yorkshire, England.
Transplantation Proceedings
|July 13, 2010
Summary
Phosphate-buffered sucrose for the heart (PBSH) solution effectively preserves rat hearts during cold ischemia. PBSH demonstrates comparable hemodynamic function and improved biochemical markers compared to established preservation solutions.
Area of Science:
- Cardiovascular Research
- Organ Preservation
- Biochemistry
Background:
- Phosphate-buffered sucrose (PBSH) solution shows promise for organ preservation.
- This study evaluates a refined PBSH for cardiac preservation.
Purpose of the Study:
- To compare the efficacy of PBSH with widely used preservation solutions (UW, St. Thomas', Celsior) for heart preservation.
- To assess hemodynamic function and biochemical markers of preserved hearts.
Main Methods:
- Rat hearts underwent cold storage (6 hours at 4°C) in PBSH or control solutions.
- Hemodynamic parameters were measured post-preservation.
- Enzyme (CPK, LDH) and metabolite (ATP, lactate, CP) levels were analyzed.
Main Results:
- Hearts preserved in PBSH exhibited comparable hemodynamic recovery to controls.
- PBSH-preserved hearts showed lower enzyme and lactate levels.
- Higher levels of energy compounds (ATP, CP) were observed in PBSH-preserved hearts.
Conclusions:
- PBSH is as effective as current standard solutions for short-term (6-hour) cold heart preservation in a rat model.
- PBSH offers potential advantages in maintaining biochemical integrity during cardiac preservation.
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