Related Experiment Video

Updated: Jun 11, 2026

Multiplex Immunohistochemistry Staining for Paraffin-embedded Lung Cancer Tissue
05:00

Multiplex Immunohistochemistry Staining for Paraffin-embedded Lung Cancer Tissue

Published on: November 21, 2023

The role of microRNA in human lung squamous cell carcinoma

Ye Yang1, Xiaofei Li, Qi Yang

  • 1Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.

Cancer Genetics and Cytogenetics
|July 13, 2010
PubMed

Insights

MicroRNAs (miRNAs) play a role in lung squamous cell carcinoma (SCC). This study found specific miRNAs were significantly downregulated or upregulated in Chinese lung SCC tissues, suggesting their involvement in cancer development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression.
  • Aberrant miRNA expression is implicated in various cancers, including lung cancer.

Purpose of the Study:

  • To investigate the expression profiles of microRNAs in Chinese lung squamous cell carcinoma (SCC).
  • To identify specific miRNAs that are deregulated in lung SCC and may contribute to its development.

Main Methods:

  • MicroRNA expression profiling was performed on 711 human miRNA genes using a miRCURY LNA array.
  • Microarray results were validated using quantitative real-time polymerase chain reaction (qRT-PCR).

Main Results:

  • Seven miRNAs (miR-126, miR-193a-3p, miR-30d, miR-30a, miR-101, let-7i, miR-15a) were significantly downregulated in lung SCC compared to normal tissues.
  • Two miRNAs (miR-185* and miR-125a-5p) were significantly upregulated in lung SCC.
  • let-7i and miR-126 showed downregulation in a high percentage of SCC samples (9/20 and 16/20, respectively).

Conclusions:

  • The observed deregulation of specific microRNAs in lung SCC suggests their potential involvement in the pathogenesis of this cancer.
  • These findings highlight the potential of certain miRNAs as biomarkers or therapeutic targets in lung SCC.

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...