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Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

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A Piglet Model of Neonatal Hypoxic-Ischemic Encephalopathy
10:30

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Published on: May 16, 2015

Angiostatins decrease in the kidney of newborn piglets after hypoxia-reoxygenation.

Marwan Emara1, Laila Obaid, Scott Johnson

  • 1Department of Oncology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2.

European Journal of Pharmacology
|July 13, 2010
PubMed
Summary
This summary is machine-generated.

Neonatal resuscitation with 100% oxygen decreased kidney angiostatin and plasminogen levels in piglets after hypoxia-reoxygenation. This may impact neonatal kidney injury and future renal development.

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Area of Science:

  • Neonatal physiology
  • Renal medicine
  • Biochemistry

Background:

  • Expression of kidney angiostatin in neonatal hypoxia-reoxygenation is not well understood.
  • Angiostatin, a key angiogenesis inhibitor, plays a role in various physiological processes.
  • Hypoxia-reoxygenation injury is a significant concern in neonates, potentially affecting organ development.

Purpose of the Study:

  • To investigate the impact of different reoxygenation concentrations (21% vs. 100% oxygen) on kidney angiostatin levels in newborn piglets subjected to hypoxia-reoxygenation.
  • To examine the correlation between angiostatin isoforms and related factors like plasminogen.
  • To assess the long-term effects on kidney tissue composition and potential implications for renal health.

Main Methods:

  • Newborn piglets underwent 2 hours of hypoxia followed by 1 hour of reoxygenation with either 21% or 100% oxygen.
  • Kidney tissue was analyzed for angiostatin isoforms (38, 43, 50kDa), total angiostatin, plasminogen, MMP-2, MMP-9, and HIF-1alpha.
  • Measurements were taken on day 4 of recovery, comparing experimental groups to sham-operated controls.

Main Results:

  • Three angiostatin isoforms (38, 43, 50kDa) were identified, with 38kDa being the predominant form.
  • The 38kDa angiostatin isoform showed significant correlation with total angiostatin and plasminogen levels.
  • On day 4, 100% reoxygenation led to decreased kidney plasminogen and angiostatin (38, 43kDa) levels compared to controls, unlike the 21% group.
  • No significant changes were observed in 50kDa angiostatin, MMP-2, MMP-9, or HIF-1alpha across groups.

Conclusions:

  • Neonatal resuscitation with 100% oxygen significantly reduces kidney plasminogen and angiostatin levels post-hypoxia-reoxygenation in piglets.
  • These alterations may contribute to neonatal kidney injury and affect long-term renal development.
  • The findings highlight the importance of oxygen concentration during neonatal resuscitation for preserving renal health.