Related Experiment Video
Updated: Jun 11, 2026

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro
Published on: August 14, 2011
Inhibitory effect on the tobacco mosaic virus infection by a plant RING finger protein
Yasuyuki Yamaji1, Koji Hamada, Toshio Yoshinuma
1Laboratory of Plant Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. ayyamaji@mail.ecc.u-tokyo.ac.jp
Abstract:
In the yeast two-hybrid screening of plant factors interacting with tobacco mosaic virus (TMV) RNA-dependent RNA polymerase (RdRp), we found a protein containing a RING finger motif in tobacco (Nicotiana tabacum) and designated it as TARF (TMV-associated RING finger protein). TARF is a homologue of a Lotus japonicus RING finger protein (LjnsRING) involved in the symbiotic interaction between L. japonicus and Mesorhizobium loti. When TARF was silenced by virus-induced gene silencing (VIGS) method, TMV RNA accumulation as well as the number of foci formed by GFP-tagged TMV increased drastically. Transient overexpression of TARF reduced the accumulation of TMV. Moreover, TARF transcription was rapidly upregulated by the inoculation of TMV in tobacco plants. These results indicated that TARF is a RING finger protein that inhibits the accumulation of TMV via the interaction of TMV RdRp.
Related Concept Videos
Inhibitors Of Virion Release
Inhibitors of Viral Protein Synthesis
Microbe-Plant Interactions
Inhibitors of Virion Maturation and Assembly
Introduction to Plant Diversity

