Antioxidant and free radical-scavenging activity of the extracellular death factor in Escherichia coli
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Updated: Jun 11, 2026

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Published on: October 23, 2016
Yanfeng Gao1, Kun Chen, Bin Zhang
1Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China. gaoyf@zzu.edu.cn
Abstract:
The extracellular death factor (EDF) is a newly discovered linear pentapeptide with the sequence of H-Asn-Asn-Trp-Asn-Asn-OH (NNWNN). It could act as a quorum-sensing molecule and participate in the mazEF-mediated cell death in Escherichia coli. In the present study, we firstly studied the antioxidant and free radical-scavenging activities of EDF. EDF could scavenge hydroxyl radicals in vitro and protect protein, lipid and DNA from being damaged by hydroxyl radicals. Our results indicated that this extracellular death factor might have dual effects during the programmed cell death process of Escherichia coli.
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