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APP(DeltaNL695) expression in murine tissue downregulates CNBP expression
Dana M Niedowicz1, Tina L Beckett, Chris J Holler
1Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY, USA.
Neuroscience Letters
|July 13, 2010
Summary
Overexpression of amyloid precursor protein (APP) reduces cellular nucleic acid binding protein (CNBP) expression in mouse models. This finding offers new insights into APP's role in neurodegenerative diseases and CNBP regulation.
Area of Science:
- Molecular Biology
- Neuroscience
- Genetics
Background:
- Cellular nucleic acid binding protein (CNBP) regulates transcription and translation and is implicated in type 2 myotonic dystrophy.
- Alzheimer's disease (AD) and sporadic inclusion body myositis (sIBM) are age-related diseases linked to beta-amyloid accumulation.
- Mouse models overexpressing amyloid precursor protein (APP) are used to study AD and sIBM.
Purpose of the Study:
- To investigate the effect of APP overexpression on CNBP expression in mouse models relevant to neurodegenerative diseases.
- To explore the potential link between APP and CNBP in the context of disease pathology.
Main Methods:
- Overexpression of APP in skeletal muscle of sIBM mouse models.
- Analysis of CNBP expression in brain-specific APP-overexpressing mice.
- Examination of CNBP expression in a whole-body APP knock-in mouse strain.
Main Results:
- Overexpression of APP in skeletal muscle of sIBM models significantly reduced CNBP expression.
- APP(Swe) expression in murine tissues, including brain and whole body, led to decreased CNBP levels.
- The reduction in CNBP expression was not attributed to transcriptional changes.
Conclusions:
- APP(Swe) expression induces a decrease in CNBP expression in mouse tissues.
- This interaction may serve as a model for studying CNBP regulation.
- Findings suggest potential roles for both APP and CNBP in the pathogenesis of age-related degenerative diseases.
