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Updated: Jun 11, 2026

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
Published on: September 10, 2018
Autocrine IL-6 regulates GRO-alpha production in thymic epithelial cells
Yau-Lin Tseng1, Ming-Ho Wu, Hsin-Chiao Yang
1Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. tsengyl@mail.ncku.edu.tw
Abstract:
Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-alpha (GRO-alpha) was subsequently expressed after IL-6 production. Either IL-6 or GRO-alpha was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-alpha production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-alpha production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-alpha and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.
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