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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

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Related Experiment Video

Updated: Jun 11, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

p53 isoforms gain functions.

V Olivares-Illana1, R Fåhraeus

  • 1INSERM Cibles Therapeutiques, Institut de Génétique Moléculaire, Hôpital St Louis, Paris, France.

Oncogene
|July 13, 2010
PubMed
Summary
This summary is machine-generated.

Different p53 isoforms mediate distinct cellular responses to stress. Understanding these p53 variants, like p53/47 and Δ133p53, is key to differentiating stress signals and tailoring cellular outcomes.

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

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Last Updated: Jun 11, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Yeast As a Chassis for Developing Functional Assays to Study Human P53

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Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cellular stress pathways converge on the p53 protein to regulate critical responses like cell cycle arrest, senescence, and apoptosis.
  • A key question is how cells distinguish between various stresses to elicit appropriate p53-mediated responses.
  • The p53 gene is known to produce multiple isoforms with distinct functional properties.

Purpose of the Study:

  • To elucidate the specific roles of different p53 isoforms in mediating cellular responses to diverse stress conditions.
  • To understand how alternative expression of p53 variants allows cells to differentiate between various stress signals.
  • To explore the evolutionary relationship between p53 pathway components and specific p53 domains.

Main Methods:

  • Analysis of alternative mRNA translation and splicing events producing p53 isoforms.
  • Investigating the functional consequences of p53 isoform expression in response to specific cellular stresses, such as endoplasmic reticulum stress.
  • Comparing the effects of different p53 isoforms (e.g., p53/47, Δ133p53, p53β) on cell cycle progression and senescence.

Main Results:

  • The p53/47 isoform, lacking the first trans-activation domain, is specifically linked to endoplasmic reticulum stress and induces a G2 arrest.
  • The canonical p53 protein induces G1 arrest but does not affect G2 arrest.
  • Isoforms Δ133p53 and p53β, generated through alternative promoter usage or splicing, are implicated in the control of cellular senescence.

Conclusions:

  • Differential expression of p53 isoforms, achieved through various gene expression control mechanisms, enables cells to distinguish between p53 activation triggers and mounting appropriate stress responses.
  • This isoform-specific regulation provides a mechanism for fine-tuning cellular fate decisions under stress.
  • Further research into p53 isoforms promises a deeper understanding of p53 pathway evolution and stress response specificity.