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Published on: February 20, 2021
The effects of lazaroid U-74389G in a rat sepsis model
Yun-Te Chang1, Shue-Ren Wann, Kai-Hseng Hsieh
1Department of Emergency Medicine, Kaohsiung Veterans General Hospital, 386 Ta-Chung 1st Road, Kaohsiung City, Taiwan.
Objective And Design:
To examine the protective effects of a lazaroid, 21-aminosteroid U-74389G, in a rat septic shock model.
Materials Or Subjects:
Male Sprague-Dawley rats (n = 60) aged 6-8 months.
Treatment:
Groups were exposed to 500 cGy radiation followed by E. coli inoculation, and either placebo or lazaroid injection (10 mg/kg intraperitoneal) 5 days after irradiation.
Methods:
Hemodynamic measurements, arterial blood gases, serum lactate, total antioxidative capacity, and cytokine levels were measured at specific time intervals.
Results:
Treatment with the lazaroid U-74389G maintained cardiac output and mean aortic pressure. Lazaroid treatment also prevented the increase in serum lactate seen in placebo-treated rats. Cytokine serum levels in lazaroid-treated rats were not significantly different from those in placebo-treated rats at any time point.
Conclusions:
Lazaroid treatment of E. coli-inoculated septic animals lessens the hemodynamic deterioration seen in sepsis.