Aptatag-based multiplexed assay for protein detection by surface-enhanced Raman spectroscopy

Area of Science:

• Nanotechnology
• Biochemistry
• Analytical Chemistry

Background:

• Protein detection assays are crucial for diagnostics.
• Existing methods often face limitations in sensitivity and selectivity.
• Nanomaterials offer potential for enhanced bioassay performance.

Purpose of the Study:

• To develop a highly sensitive and selective multiplexed bioassay for protein detection.
• To utilize silver-nanoparticle dimers as a core component for the assay.
• To demonstrate the efficacy of DNA aptamers and polyethylene glycol for assay stabilization and targeting.

Main Methods:

• Fabrication of silver-nanoparticle dimers functionalized with Raman reporters.
• Capping the dimers with DNA aptamers for specific protein recognition.
• Stabilization of the nanostructures using polyethylene glycol chains.
• Development of a multiplexed heterogeneous bioassay format.

Main Results:

• The developed bioassay demonstrated high sensitivity in protein detection.
• The assay exhibited excellent selectivity, distinguishing target proteins effectively.
• The multiplexed format allowed for simultaneous detection of multiple proteins.

Conclusions:

• Silver-nanoparticle dimers functionalized with aptamers provide a robust platform for sensitive protein detection.
• The assay design offers a promising approach for multiplexed diagnostics.
• This method holds potential for advancements in biomarker discovery and clinical analysis.