Related Experiment Video
Updated: Jun 11, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Published on: March 7, 2019
What we learn from transformation suppressor genes: lessons from RECK.
Makoto Noda1, Chiaki Takahashi, Tomoko Matsuzaki
1Department of Molecular Oncology & Global COE Program, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. mnoda@virus.kyoto-u.ac.jp
Expression cloning identifies cancer-driving genes by reverting transformed cells. Validating these genes
Area of Science:
- Oncology and Molecular Biology: Focuses on gene function in cancer development and progression.
Background:
- Expression cloning is a method for discovering genes that alter cell characteristics.
- Identifying genes that reverse cancer cell transformation is a key application in cancer research.
- Clinical relevance of genes identified through artificial screening requires independent validation.
Purpose of the Study:
- To explore the potential and limitations of expression cloning in cancer research.
- To discuss recent advancements in expression cloning techniques.
- To highlight the importance of validating identified genes for clinical applications, using RECK as an example.
Main Methods:
- Utilizing expression cloning to isolate cDNAs that induce flat reversion in transformed cells.
- Discussing the process of independent validation for clinical relevance.
- Examining the biological mechanisms, functions, and regulation of identified genes.
Main Results:
- Expression cloning can identify genes with potential roles in cancer suppression.
- The RECK gene, a transformation suppressor, serves as a case study for this approach.
- Further research into identified genes can enhance understanding of cancer biology.
Conclusions:
- Expression cloning is a valuable tool in cancer research for identifying potential therapeutic targets.
- Independent validation is crucial to confirm the clinical significance of genes discovered through this method.
- Understanding gene mechanisms and regulation is vital for developing novel cancer diagnostics and therapies.
More Related Videos
07:53Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
Published on: January 1, 2018
05:28Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
Published on: June 2, 2023
Related Concept Videos
In-vitro Mutagenesis
Overview of Transposition and Recombination
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...