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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Therapeutic antibodies: Discovery and development using the ProteOn XPR36 biosensor interaction array system.

Vered Bronner1, Galit Denkberg, Mira Peled

  • 1Bio-Rad Laboratories, Gutwirth Park, Technion, Haifa 32000, Israel.

Analytical Biochemistry
|July 14, 2010
PubMed
Summary

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This study introduces the ProteOn XPR36 system for rapid, high-throughput screening of therapeutic monoclonal antibodies. It efficiently selects high-affinity antibody candidates by providing key binding constants simultaneously.

Area of Science:

  • Biotechnology
  • Immunology
  • Pharmaceutical Science

Background:

  • Therapeutic monoclonal antibodies represent a rapidly expanding pharmaceutical class.
  • Efficient screening and selection of high-affinity, specific antibodies are crucial for drug development.
  • Existing methods can be time-consuming for high-throughput screening.

Purpose of the Study:

  • To demonstrate the utility of the ProteOn XPR36 system with its One-Shot Kinetics approach.
  • To showcase rapid screening and selection of high-affinity therapeutic antibodies.
  • To validate the system for various stages of antibody discovery and development.

Main Methods:

  • Utilized the ProteOn XPR36 protein interaction array system.
  • Employed the One-Shot Kinetics methodology for parallel binding analyses.

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  • Quantitatively determined association, dissociation, and affinity constants simultaneously.

    • Main Results:

    • The ProteOn XPR36 system enabled rapid screening of multiple antibodies or supernatants in a single experiment.
    • Simultaneous determination of kinetic and affinity constants was achieved.
    • Demonstrated efficient antibody selection with high affinity and specificity.

    Conclusions:

    • The ProteOn XPR36 system is a valuable tool for therapeutic antibody discovery and development.
    • The One-Shot Kinetics approach significantly accelerates screening and selection processes.
    • The system supports multiple stages, including post-panning, affinity maturation, and clone selection.