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Updated: Jun 11, 2026

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
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Selection and versatile application of virus-specific aptamers.

Zsófia Balogh1, Gergely Lautner, Viola Bardóczy

  • 1Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|July 14, 2010
PubMed
Summary

Researchers developed novel aptamers as alternatives to antibodies for apple stem pitting virus (ASPV) detection. These aptamers offer a more sensitive, flexible, and cost-effective method for plant virus diagnostics using enzyme-linked oligonucleotide assay (ELONA).

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Area of Science:

  • Plant Pathology
  • Molecular Diagnostics
  • Biotechnology

Background:

  • Traditional antibody-based immunoassays are standard for plant virus detection.
  • Aptamers are emerging as promising alternatives to antibodies in various diagnostic applications.
  • There is a need for improved diagnostic tools for plant viruses like apple stem pitting virus (ASPV).

Purpose of the Study:

  • To select ASPV-specific aptamers as potential antibody substitutes.
  • To develop and validate aptamer-based immunoassay protocols for plant virus diagnosis.
  • To evaluate the performance of aptamers compared to antibodies in detecting ASPV.

Main Methods:

  • Systematic Evolution of Ligands by Exponential Enrichment (SELEX) was used to select aptamers.
  • Aptamers were applied in dot blot, Western blot, and enzyme-linked oligonucleotide assay (ELONA).

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  • Assay performance was compared between aptamers and conventional antibodies.

    • Main Results:

    • Highly discriminative aptamers were selected, specifically binding to the ASPV coat protein.
    • Aptamers demonstrated superior performance over antibodies, detecting both native and denatured proteins.
    • ELONA using aptamers yielded higher signal intensity than ELISA with infected plant extracts.

    Conclusions:

    • The study presents the first selection and application of plant virus-specific aptamers.
    • Aptamer-based ELONA offers a novel, flexible, and cost-effective method for plant virus diagnostics.
    • These findings pave the way for advanced molecular diagnostic tools in agriculture.