Related Experiment Video

Updated: Jun 11, 2026

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down
08:59

Looking for Driver Pathways of Acquired Resistance to Targeted Therapy: Drug Resistant Subclone Generation and Sensitivity Restoring by Gene Knock-down

Published on: December 11, 2017

iTRAQ quantitative analysis of multidrug resistance mechanisms in human gastric cancer cells

Huai-Dong Hu1, Feng Ye, Da-Zhi Zhang

  • 1Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.

Journal of Biomedicine & Biotechnology
|July 14, 2010
PubMed

Insights

This study investigated multidrug resistance (MDR) in gastric cancer using proteomic analysis. We identified 91 differentially expressed proteins, including MVP, offering new insights into gastric cancer drug resistance mechanisms.

Area of Science:

  • Proteomics
  • Molecular Biology
  • Oncology

Background:

  • Multidrug resistance (MDR) presents a significant challenge in gastric cancer treatment.
  • The molecular mechanisms underlying gastric cancer MDR remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanisms of MDR in gastric cancer.
  • To identify novel protein biomarkers associated with MDR in gastric cancer.

Main Methods:

  • Proteomic analysis using isobaric tags for relative and absolute quantification (iTRAQ) coupled with LC-MS/MS.
  • Utilized vincristine-resistant (SGC7901/VCR) and parental (SGC7901) gastric cancer cell lines as a model.
  • Western blot analysis for validation of differentially expressed proteins.

Main Results:

  • Identified 820 unique proteins, with 91 proteins exhibiting differential expression between resistant and parental cell lines.
  • Confirmed the association of Multiviscin-like Protein 1 (MVP) with MDR in gastric cancer.
  • Several novel proteins linked to MDR were discovered.

Conclusions:

  • This study represents the first application of iTRAQ technology to analyze MDR mechanisms in gastric cancer.
  • The identified differentially expressed proteins, particularly MVP, provide valuable targets for understanding and potentially overcoming gastric cancer MDR.
  • Highlights the utility of advanced proteomic techniques in cancer research.

More Related Videos

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance05:23

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance

Published on: March 7, 2025

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...