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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 11, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Single-particle tracking photoactivated localization microscopy for mapping single-molecule dynamics.

Suliana Manley1, Jennifer M Gillette, Jennifer Lippincott-Schwartz

  • 1Institute of Physics of Biological Systems, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland.

Methods in Enzymology
|July 15, 2010
PubMed
Summary

Single-molecule tracking photoactivated localization microscopy (sptPALM) offers high-resolution insights into cellular dynamics. This technique analyzes individual molecule motion, advancing our understanding of biological processes.

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Related Experiment Videos

Last Updated: Jun 11, 2026

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
12:42

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Published on: December 22, 2015

Area of Science:

  • Cellular biology
  • Biophysics
  • Microscopy

Background:

  • Single-molecule localization microscopy (SMLM) enables high-resolution analysis of molecular dynamics.
  • Photoactivatable fluorescent proteins (PAFPs) are crucial for SMLM techniques.
  • Understanding molecular motion is key to deciphering cellular mechanisms.

Purpose of the Study:

  • To describe the single-molecule tracking photoactivated localization microscopy (sptPALM) method.
  • To highlight the capabilities of sptPALM for studying cellular processes.
  • To promote a quantitative understanding of fundamental cellular dynamics.

Main Methods:

  • Utilizing photoactivatable fluorescent proteins for single-molecule tracking.
  • Employing sptPALM for high spatial (nanometer) and temporal (millisecond) resolution.
  • Analyzing the motion of individual molecules within living cells.

Main Results:

  • Achieved high specificity, millisecond time resolution, and nanometer spatial resolution in probing single-molecule motion.
  • Demonstrated the potential for analyzing high-density molecular dynamics.
  • Provided a framework for quantitative analysis of cellular processes.

Conclusions:

  • sptPALM is a powerful technique for investigating molecular dynamics in living cells.
  • This method offers new insights into protein heterogeneity, cytoskeletal flow, and receptor clustering.
  • sptPALM contributes to a quantitative understanding of fundamental cellular processes.