Single-molecule dual-beam optical trap analysis of protein structure and function
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Updated: Jun 11, 2026

06:53
Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
Published on: November 18, 2022
Jongmin Sung1, Sivaraj Sivaramakrishnan, Alexander R Dunn
1Department of Biochemistry, Stanford University School of Medicine, Stanford, California, USA.
Methods in Enzymology
|July 15, 2010
Abstract:
Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.

