Related Experiment Video
Updated: Jun 11, 2026

09:58
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
Published on: December 9, 2016
Biological Sequence Mining Using Plausible Neural Network and its Application to Exon/intron Boundaries Prediction
Kuochen Li1, Dar-Jen Chang, Eric Rouchka
1CECS, University of Louisville, Louisville, KY 40292, USA.
Summary
This study introduces the Plausible Neural Network (PNN) for efficient biological sequence mining. PNN offers a unified approach for analyzing large datasets, demonstrating its capability in tasks like exon/intron prediction.
Area of Science:
- Bioinformatics
- Computational Biology
- Machine Learning
Background:
- Biological sequence data analysis is computationally intensive.
- Mining large biological datasets presents challenges in pattern discovery, classification, and clustering.
- Existing methods often require significant computation time.
Purpose of the Study:
- Introduce the Plausible Neural Network (PNN) as a novel model for biological sequence mining.
- Demonstrate the application of PNN in analyzing large biological sequence datasets.
- Evaluate PNN's effectiveness for specific biological sequence mining tasks.
Main Methods:
- The paper details the fundamental concepts of the Plausible Neural Network (PNN).
- PNN's architecture is presented as an intuitive and unified framework.
- The model is applied to the task of exon/intron prediction in biological sequences.
Main Results:
- Experimental results validate the efficacy of PNN for biological sequence mining.
- PNN successfully addresses the challenges of large dataset analysis.
- The model proves capable of performing complex sequence analysis tasks.
Conclusions:
- The Plausible Neural Network (PNN) is a capable tool for biological sequence mining.
- PNN offers an efficient and unified solution for analyzing large biological datasets.
- The model shows promise for various applications in bioinformatics and computational biology.
Related Concept Videos
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Signal Sequences and Sorting Receptors
Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Multi-species Conserved Sequences
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
