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[Cardioprotective effects of St. Thomas solution containing PolyHb on isolated rat heart during cold preservation]
1Department of Pharmacology & Biopharmaceutics, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the cardioprotective effects of St. Thomas solution containing PolyHb on ischemia-reperfusion injury to isolated rat heart after eight-hour cold storage.
Methods:
Sixty male inbred SD rats were randomly divided into 3 groups (n = 20 in each group): sham group, St. Thomas solution (STS) group and STS with PolyHb group. The Langendoff isolated rat heart model was used. The hearts were stored in 4 degrees C St. Thomas solution with or without PolyHb for 8 h followed by 120 min of reperfusion. The recovery of cardiac function was observed. Myocardial enzyme leakage and infarct size were measured after reperfusion.
Results:
In comparison with STS group, St. Thomas solution containing PolyHb improved the recovery of LVDP (P < 0.001) and +/- dp/dt (P < 0.001), decreased the release of CK and LDH (P < 0.001), reduced the percentage of the infarct size (P < 0.001) of the heart during reperfusion as well.
Conclusion:
Adding PolyHb to St. Thomas solution can protect myocardium from ischemia-reperfusion injury after eight-hour cold preservation.
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