[Determination of avermectin and ivermectin in swine muscular tissues using high performance liquid
Zhi-Xiong Wu1, Jiang-Tao Hu, Wei-Dong Zheng
1Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To develop a method for simultaneous determination of avermectin (AVM) and ivermectin (IVM) in swine muscular tissues using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
Methods:
The AVM and IVM were extracted with acetonitrile, cleaned with basic alumina solid phase extraction cartridges, separated through C8 column, and then detected by mass spectrometry in positive ion mode with multiple reaction monitoring (MRM) using an electrospray ionization interface, with quantitative ion pair of 890.4/305.3 and 892.5/307.3 for AVM and IVM, respectively.
Results:
The linear ranges for both AVM and IVM were between 5 microg/L and 100 microg/L, with a correlation coefficient of 0.9999. The detection limit was 2.0 microg/kg. The recoveries at the spiked concentration of 2 microg/kg, 10 microg/kg and 20 microg/kg ranged from 77.4% to 83.1% for AVM and 77.2% to 84.8% for IVM, with relative standard deviation (RSD) ranging from 8.7% to 11.7% for AVM and 6.3% to 10.3% for IVM, respectively. No AVM and IVM residues were detected in samples taken from Chengdu markets.
Conclusion:
A HPLC-MS/MS method was successfully developed for determining AVM and IVM in swine muscular tissues, which is simple, sensitive and precise and can meet both domestic and international requirements.
More Related Videos
09:26Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
08:53Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Published on: May 7, 2018
Related Concept Videos
MALDI-TOF Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
