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Updated: Jun 10, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Evaluation of human osteosarcoma cell line genotoxicity effects of mineral trixoide aggregate and calcium silicate
Shinn-Jyh Ding1, Chia-Tze Kao, Chih-Lin Chen
1Institute of Oral Biology and Biomaterials, Chung Shan Medical University, Taichung, Taiwan.
Introduction:
Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63).
Methods:
A mitochondrial colorimetric assay was used to evaluate the MG63 survival rate. A DNA precipitation assay was used to detect the MG63 DNA damage after contact with MTA or CS cement extracts.
Results:
The results showed that MTA and CS are cytocompatible with MG63 cells. There was no significant difference in the survival rate with MTA and CS materials (P > .05). Neither MTA nor CS cements causing MG63 cell DNA damage showed significant genotoxicity (P > .05).
Conclusions:
Both MTA and CS cements are compatible with MG63 cells, and they are not cancer-causing agents.
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