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Related Experiment Video

Updated: Jun 10, 2026

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Published on: August 14, 2014

Endothelial f-actin depolymerization enables leukocyte transmigration.

Laura Isac¹, Gerold Thoelking, Albrecht Schwab

¹Institute of Physiology II, University of Münster, Germany.

Analytical and Bioanalytical Chemistry

|July 16, 2010

Summary

This summary is machine-generated.

Leukocytes dramatically rearrange endothelial cytoskeleton during diapedesis, making the cell softer and depolymerizing f-actin to create transmigratory channels for immune defense.

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Last Updated: Jun 10, 2026

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

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Published on: August 14, 2014

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Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Published on: April 9, 2018

Area of Science:

Immunology and Cell Biology
Investigating cellular immune responses and leukocyte trafficking.

Background:

Leukocytes are key to host defense, migrating from blood to tissues via diapedesis.
Endothelial cells control leukocyte emigration, but the mechanism of diapedesis remains unclear.
Leukocytes can move paracellularly or transcellularly through endothelial cells.

Purpose of the Study:

To elucidate the mechanism of endothelial cell transmigration during diapedesis.
To investigate the role of the endothelial cytoskeleton in forming transmigratory channels.

Main Methods:

Utilized Atomic Force Microscopy (AFM) to probe endothelial cell mechanics.
Applied a novel
nanosurgery
protocol for direct visualization of leukocyte-endothelial interactions.
Employed Fluorescence Microscopy to observe cytoskeletal dynamics.

Main Results:

Discovered that endothelial cells soften in a localized region beneath adhering leukocytes.
Confirmed f-actin depolymerization at the leukocyte invasion site.
Demonstrated that leukocytes actively remodel the endothelial cytoskeleton to facilitate transcellular diapedesis.

Conclusions:

Leukocyte diapedesis involves significant remodeling of the endothelial cytoskeleton.
Endothelial cell softening and f-actin depolymerization are critical for forming transmigratory channels.
This study provides new insights into the cellular mechanisms governing immune cell trafficking.