Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Radiogenic free radicals as molecular probes in bone.

R A Peckauskas, I Pullman

    Calcified Tissue Research
    |February 28, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Electron spin resonance studies on tooth phosphoproteins using manganese ions as probes.

    Archives of biochemistry and biophysics·1979
    Same author

    Radiation induced free radicals as molecular probes in synthetic apatites.

    Calcified tissue research·1978
    Same author

    ESR investigation of the binding of some neutral polyamino acids to synthetic apatite.

    Biopolymers·1977
    Same author

    Radiogenic free radicals in apatite: the influence of fluoride and hydroxide.

    Calcified tissue research·1976
    Same author

    ESR investigation of the binding of acidic biopolymers to synthetic apatite.

    Biopolymers·1976
    Same author

    Nitrate radicals in apatites.

    Journal of dental research·1975

    X-rays create unstable free radicals in bone mineral. Electron spin resonance spectroscopy at low temperatures identified electron excess and deficit centers, primarily on the mineral surface.

    Area of Science:

    • Biophysics
    • Materials Science
    • Radiology

    Background:

    • Bone mineral is susceptible to radiation damage.
    • Free radicals are highly reactive species generated by X-ray exposure.
    • Studying these radicals provides insight into bone material properties and radiation interactions.

    Purpose of the Study:

    • To characterize free radicals generated in bone mineral by X-ray exposure.
    • To investigate the localization and environmental sensitivity of these radicals.
    • To compare bone mineral with synthetic analogs for radiation studies.

    Main Methods:

    • X-ray irradiation of bone mineral samples.
    • Stabilization of free radicals at liquid nitrogen temperatures.
    • Analysis using electron spin resonance (ESR) spectroscopy.

    Related Experiment Videos

    Main Results:

    • Identified one electron excess center and two electron deficit centers (phosphate anion radical, carbonate-associated radical).
    • Radicals were predominantly located on the mineral surface and sensitive to the surface environment.
    • The presence of an organic phase (whole bone) altered free radical production, suggesting interference with electron deficit center formation.

    Conclusions:

    • Precipitated carbonate-apatites serve as effective models for bone mineral in radiation studies.
    • Understanding free radical formation is crucial for assessing bone's response to radiation.
    • Surface properties significantly influence radiation-induced radical generation in bone mineral.