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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 10, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Make them blink: probes for super-resolution microscopy.

Jan Vogelsang¹, Christian Steinhauer, Carsten Forthmann

¹Center for Nano and Molecular Science and Technology and Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry

|July 16, 2010

Summary

This summary is machine-generated.

Super-resolution microscopy breaks the light diffraction limit for biological imaging. This review covers techniques and fluorescent probes, showing how standard fluorophores enable advanced imaging and calibration.

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Last Updated: Jun 10, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 28, 2018

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Published on: September 6, 2013

Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Area of Science:

Biophysics
Optical Microscopy
Nanotechnology

Background:

Far-field fluorescence imaging traditionally limited by light diffraction.
Super-resolution microscopy (SRM) offers enhanced resolution for biological structures.
SRM techniques are becoming accessible to conventional labs.

Purpose of the Study:

Provide an overview of recent SRM developments.
Outline requirements for fluorescent probes in SRM.
Demonstrate the use of standard fluorophores in SRM.

Main Methods:

Categorization of SRM techniques (targeted vs. stochastic switching).
Analysis of photophysics and photochemistry of single fluorophores.
Application of standard organic fluorophores in SRM.
Development of calibration samples using AFM and DNA origami.

Main Results:

SRM techniques enable imaging beyond the diffraction limit.
Any single-molecule compatible fluorophore can be utilized for SRM.
Factors influencing resolution were discussed.
Novel calibration sample preparation methods were presented.

Conclusions:

SRM significantly advances biological structure and dynamics visualization.
Understanding fluorophore photophysics is key for SRM implementation.
Standard fluorophores and advanced calibration methods broaden SRM accessibility.