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Related Experiment Video

Updated: Jun 10, 2026

Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells
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Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells

Published on: May 31, 2024

[In vitro quantum dot-labeled rat bone marrow mesenchymal stem cells].

Xiaorui Jiang1, Xinxin Zhang, Keyue Yang

  • 1Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou Guangdong, 510515, P.R. China

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery
|July 17, 2010
PubMed
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Quantum dot 655 (QD655) is a safe and effective marker for labeling rat bone marrow mesenchymal stem cells (BMSCs). This study confirms QD655

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Nanotechnology

Background:

  • Rat bone marrow mesenchymal stem cells (BMSCs) are crucial for regenerative medicine.
  • Effective labeling methods are needed for tracking BMSCs in vivo.
  • Quantum dots offer unique fluorescent properties for cellular imaging.

Purpose of the Study:

  • To evaluate the safety and efficacy of Quantum Dot 655 (QD655) for labeling rat BMSCs.
  • To assess the impact of QD655 labeling on BMSC viability, proliferation, and differentiation.
  • To determine the long-term retention and cell adhesion characteristics of QD655-labeled BMSCs.

Main Methods:

  • BMSCs were isolated, cultured, and characterized.
  • Cells were labeled with QD655 at a recommended concentration.

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  • Cytotoxicity was assessed using trypan-blue exclusion.
  • Cell proliferation was measured by MTT assay.
  • Osteogenic differentiation was evaluated via Alizarin red staining, ALP staining, and qRT-PCR.
  • Labeling efficiency and cell adhesion to a bioglass/collagen scaffold were observed using fluorescence and scanning electron microscopy over 6 weeks.

    • Main Results:

    • QD655 labeling did not significantly affect BMSC survival or proliferation (P > 0.05).
    • Labeled BMSCs retained osteogenic differentiation potential.
    • High labeling efficiency was observed, with rates ranging from 96.5% at 0 weeks to 10% at 6 weeks.
    • QD655-labeled BMSCs exhibited good adhesion to the scaffold.

    Conclusions:

    • QD655 is a safe and effective fluorescent marker for labeling rat BMSCs.
    • QD655 labeling does not impede the multipotent differentiation capacity of BMSCs.
    • QD655 is a feasible tool for stem cell labeling and in vivo tracking.