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Updated: Jun 10, 2026
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
Published on: March 7, 2015
[Comparative study on potential of adipogenic differentiation between dedifferentiated adipocytes and adipose-derived
Yunjun Liao1, Jianhua Gao, Feng Lu
1Department of Plastic and Reconstructive Surgery, Nanfang Hospital Southern Medical University, Guangzhou Guangdong, 510515, P.R. China.
Objective:
Seed cells are the hotspot of tissue engineering research. To study the seed cells with high potential of adipogenic differentiation for applying the adipose tissue engineering and increasing the constructing efficiency of adipose tissue engineering.
Methods:
Mature adipocytes (MA) and adipose-derived stromal cells (ADSCs) were harvested from human fat aspirates via liposuction by collagenase digestion. MA were cultured and induced to dedifferentiated adipocytes (DA) by ceiling adherent culture method. DA and ADSCs were induced to adipogenic differentiation. The adipogenic abilities of DA and ADSCs were compared by inverted phase contrast microscope observation, absorption spectrometry assay of oil red O staining, and cell counting of oil red O staining.
Results:
MA could dedifferentiate into fibroblast-shaped DA. After adipogenic differentiation, the inverted phase contrast microscope observation showed that there were much more lipid droplet in DA than in ADSCs. Absorption spectrometry assay of oil red O staining showed there were significant lipid droplet aggregation in DA 4 days of adipogenic induction. However, the same phenomenon could be observed in ADSCs at 10 days after differentiation. After 12 days, the absorption value of DA was higher than that of ADSCs, showing significant difference (P < 0.05). The cell counting of oil red O staining demonstrated that the adipogenic rates of DA and ADSCs were 65% +/- 6% and 35% -/+ 5%, respectively, showing significant difference (P < 0.05).
Conclusion:
The potential of adipogenic differentiation of DA is stronger than that of ADSCs. DA is a promising seed cell of adipose tissue engineering.
