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Updated: Jun 10, 2026
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
Published on: January 26, 2019
[Isolation of mesenchymal stem cells from human umbilical cord blood and its bone inducing activity in vitro]
He Zhang1, Ang Li, Guo-zhou Rao
1Department of Orthodontics, Stomatological Hospital of Xi'an Jiaotong University, Xi'an 710004, China. bluewater1123@yeah.net
Purpose:
To investigate the biological characters of mesenchymal stem cells obtained from human umbilical cord blood(HUCB-MSCs), and detect the influence of different serum concentrations of culture medium to primary HUCB-MSCs' growth kinetics and the osteogenic differentiation capacity of the passage cells.
Methods:
The mononuclear cells were isolated from umbilical cord blood(UCB), the growth characters and morphology of the primary MSCs were observed. Primary cells were cultured in alpha-MEM with different serum concentrations, and immunophenotype was investigated. P3 cells were induced to osteoblast-like cells and the differentiation results were analysed. SPSS 16.0 software package was used for statistical analysis through repeated measures analysis of variances.
Results:
Four subpopulations with different morphology could be distinguished in primary HUCB-MSCs. All the subpopulations were positive for CD44 and CD105, and negative for CD34 and CD45. HUCB-MSCs displayed faster and stable growth when cultured in alpha-MEM containing 15% serum. Passage cells of HUCB-MSCs could be induced into osteoblast-like cells, the formation of mineralized extracellular matrix and the positive staining of expression of ALP could be observed.
Conclusions:
HUCB-MSCs are high in viability but low in proliferation. HUCB-MSCs contain 4 subpopulations with different morphology. The appropriate concentration of serum in culture medium should be 15%. HUCB-MSCs could be induced into osteoblast-like cells in vitro.
