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Immunochemical studies on a Brucella ovis specific protein antigen
C E Suarez1, G A Pacheco, A M Vigliocco
1Comision Nacional de Energia Atomica, Buenos Aires, Argentina.
Veterinary Microbiology
|April 1, 1991
Summary
Researchers identified a Brucella ovis surface protein antigen (P-II). This antigen specifically detected infection in rams caused by B. ovis, distinguishing it from other Brucella species.
Area of Science:
- Veterinary Microbiology
- Immunology
- Bacteriology
Background:
- Brucella ovis causes significant reproductive losses in rams.
- Accurate diagnostics are crucial for controlling Brucella ovis infections.
- Surface antigens are key targets for serological detection.
Purpose of the Study:
- To characterize a surface protein antigen (P-II) from Brucella ovis.
- To evaluate the specificity of P-II in detecting B. ovis infections.
Main Methods:
- Purification of P-II using Sepharose 4B gel filtration.
- Analysis of P-II molecular weight by gradient SDS-PAGE.
- Radioimmunoprecipitation assay (RIPA) with 125I-labeled P-II.
Main Results:
- P-II analysis revealed two bands at 18.5 and 20 kDa.
- P-II specifically reacted with sera from rams infected with rough B. ovis.
- No cross-reactivity was observed with sera from rams infected with smooth Brucella abortus or Brucella melitensis.
Conclusions:
- The characterized Brucella ovis P-II antigen shows high specificity.
- P-II is a promising candidate for developing diagnostic tests for B. ovis infections.
- This antigen can differentiate B. ovis from other Brucella species infections.