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Updated: Jun 10, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Single-molecule DNA analysis.

J William Efcavitch¹, John F Thompson

¹Helicos BioSciences Corporation, Cambridge, Massachusetts 02139, USA. jwefcavitch@helicosbio.com

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|July 20, 2010

Summary

This summary is machine-generated.

Single-molecule detection of DNA and RNA enables detailed studies of molecular dynamics and protein interactions. This technology significantly enhances DNA sequencing throughput, promising future advancements in biomolecular analysis.

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Last Updated: Jun 10, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

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Published on: February 7, 2021

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

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Published on: September 27, 2024

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Area of Science:

Biochemistry and Molecular Biology
Genomics and Genetics
Analytical Chemistry

Background:

Single-molecule detection offers unprecedented insights into nucleic acid properties.
Techniques involve tethering, confinement, or diffusion into detection volumes.
Applications extend beyond basic research to high-throughput DNA sequencing.

Purpose of the Study:

To review advancements in single-molecule DNA and RNA detection.
To survey efforts in molecular dynamics and nucleic acid sequencing.
To highlight the impact of single-molecule analysis on biological research.

Main Methods:

Optical and nonoptical detection methods are employed.
Techniques include surface-based, nanopore, and zero-mode waveguide approaches.
Manipulation of nucleic acids is key for examining dynamics and interactions.

Main Results:

Single-molecule detection has revolutionized DNA sequencing throughput.
Novel techniques allow detailed examination of structural dynamics.
Protein-DNA interactions can be studied at the single-molecule level.

Conclusions:

Single-molecule detection is a powerful tool for understanding nucleic acids.
Continued progress is expected in sequencing and molecular dynamics.
The field offers diverse applications in biological and medical research.